1．基本信息
王曉峰，男，陜西咸陽人，西北農(nóng)林科技大學(xué)《作物抗逆與高效生產(chǎn)國家重點(diǎn)實(shí)驗(yàn)室》研究人員，園藝學(xué)院教授、博士生導(dǎo)師。在西北農(nóng)業(yè)大學(xué)園藝系學(xué)習(xí)，先后獲學(xué)士和碩士學(xué)位，1993年7月留校任教。1999年在西北農(nóng)業(yè)大學(xué)獲博士學(xué)位。2000年至2002年在以色列農(nóng)業(yè)研究中心（Volcani Center）和以色列魏茲曼研究所（Weizmann Institute）做博士后研究，2002年至2010年在美國北卡羅來納州州立大學(xué)（NC State University）從事科研工作。
現(xiàn)為陜西省人才計(jì)劃特聘教授，陜西省政府特聘專家，陜西省園藝學(xué)會理事。
2．研究方向和研究興趣
主要從事植物生化和分子生物學(xué)的研究及其在園藝作物代謝調(diào)控分子育種上的應(yīng)用, 內(nèi)容包括植物組織培養(yǎng)及遺傳轉(zhuǎn)化，基因克隆和功能分析，植物生長發(fā)育過程中的基因表達(dá)調(diào)控，植物激素信號傳導(dǎo)的分子機(jī)制，植物信號傳導(dǎo)的功能基因組學(xué)和蛋白組學(xué)等。研究興趣主要為油菜素甾醇受體(BRI1) 及其共受體(BAK1) 的互作組分及信號傳導(dǎo)的分子機(jī)理， 膜蛋白受體的生物學(xué)功能和相互作用等，并致力于將這些基礎(chǔ)研究的成果應(yīng)用于園藝作物的栽培和育種實(shí)踐。
應(yīng)用免疫沉淀及液相色譜與質(zhì)譜連用(LC-MS/MS)，對BRI1(油菜素甾醇受體) 和BAK1(油菜素甾醇受體的共受體)的活體磷酸化位點(diǎn)進(jìn)行了系統(tǒng)的鑒定，首次在植物界建立了完整的受體激酶活體磷酸化圖譜。借助單個(gè)位點(diǎn)的定向突變對所鑒定的磷酸化位點(diǎn)的生化和生物學(xué)功能進(jìn)行了評估。通過對BRI1和BAK1復(fù)合體形成和磷酸化的研究, 以及對BRI1 和BAK1 的信號分解，揭示了油菜素甾醇信號傳導(dǎo)分子機(jī)理的幾個(gè)重要方面，建立了油菜素早期信號傳導(dǎo)模型。近年來在番茄、馬鈴薯和白菜等蔬菜作物上，通過磷酸化位點(diǎn)突變的基因的過表達(dá)和基因敲除編輯，研究油菜素甾醇路徑組分的磷酸化位點(diǎn)的農(nóng)藝學(xué)價(jià)值及其在分子育種上的應(yīng)用，以期通過人為操控磷酸化位點(diǎn)的磷酸化，精準(zhǔn)調(diào)控油菜素甾醇信號的強(qiáng)度和特異性，進(jìn)而改良作物的重要農(nóng)藝性狀。
3. 開設(shè)課程
主講課程包括《園藝植物生物技術(shù)》、《蔬菜栽培學(xué)》、《食用菌栽培學(xué)》、《新生研討課》、《科研基本方法》、《瓜果類蔬菜栽培學(xué)》、《蔬菜學(xué)研究進(jìn)展》等。
4. 主要研究論文
1.Maqsood Khan, Bote Luo, Jianwei Liu, Siwei Zhang, Dan Luo, Rui Deng, Yuchao Li, Tong Jin, Shufen Wang and Xiaofeng Wang*. (2025) Transcriptional regulation of SlFRIGIDA-like improve antioxidant system in tomato under cold. BMC Plant Biology, 25:1030, doi: 10.1186/s12870-025-06993-x
2.Siwei Zhang#, Rui Deng#, Jianwei Liu#, Dan Luo, Miaomiao Hu, Shuhua Huang, Meng Jiang, Jia Du, Tong Jin, Dehai Liu, Yuchao Li, Maqsood Khan, ShufenWang*, XiaofengWang*. (2024) Phosphorylation of the transcription factor SlBIML1 by SlBIN2 kinases delays flowering in tomato. Plant Physiology, 196: 2583–2598, doi:10.1093/plphys/kiae489
3.Rui Deng#, Shuhua Huang#, Jia Du, Dan Luo, Jianwei Liu, Yan Zhao, Chongyang Zheng, Tiantian Lei, Qi Li, Siwei Zhang, Meng Jiang, Tong Jin, Dehai Liu, Shufen Wang, Yanfeng Zhang, and Xiaofeng Wang*. (2024) The brassinosteroid receptor StBRI1 promotes tuber development by enhancing plasma membrane H+-ATPase activity in potato. The Plant Cell, 36: 3498–3520
4.Cheng Li#, Shan Zhang#, Jingjuan Li, Shuhua Huang, Tong Zhao, Siqi Lv, Jianwei Liu, Shufen Wang, Xiaohui Liu, Shen He, Yanfeng Zhang, Fangming Xiao, Fengde Wang, Jianwei Gao* and Xiaofeng Wang*. (2024) PHB3 interacts with BRI1 and BAK1 to mediate brassinosteroidsignal transduction in Arabidopsis and tomato. New Phytologist, 241: 1510–1524, doi: 10.1111/nph.19469
5.Shufen Wang*, Dehai Liu, Tong Jin, Jia Du, Aijuan Tian, Qiang Huang, Maqsood Khan, Shuming Nie, Siwei Zhang, Rui Deng, Mingke Zhang, Xiaofeng Wang*. (2023) Manipulation of brassinosteroid signaling via overexpression of its major receptor SlBRI1 regulates salt tolerance in tomato. Scientia Horticulturae, 322: 112457
6.Meng Jiang, Shangtan Fu, Xiaofeng Wang*. (2022) Application Status and Prospect of CRISPR/Cas9 Genome Editing Technology in Tomato. Acta Botanica Boreali-Occidentalia Sinica, 2022, 42(2):0348-0360
7.Maqsood Khan #, Bote Luo#, Miaomiao Hu, Shantan Fu, jianwei Liu, Meng Jiang, Yang Zhao, shuhua Huang, shufen Wang, Xiaofeng Wang,* （2022）Brassinosteroid Signaling Downstream Suppressor BIN2 Interacts with SLFRIGIDA-LIKE to Induce Early Flowering in Tomato. International Journal of molecular Sciences, 23: 11264. https://doi.org/10.3390/ijms231911264
8.Shufen Wang# , Siqi Lv# , Tong Zhao, Meng Jiang, Dehai Liu, Shangtan Fu, Miaomiao Hu, Shuhua Huang, Yu Pei and Xiaofeng Wang*. (2021) Modification of threonine-825 of SlBRI1 enlarges cell size to enhance fruit yield by regulating the cooperation of BR-GA signaling in tomato. International Journal of molecular Sciences, 22: 7673. https://doi.org/10.3390/ijms22147673
9.Shuhua Huang, Chongyang Zheng, Yan Zhao, Qi Li, Jianwei Liu, Rui Deng, Tiantian Lei, Shufen Wang, Xiaofeng Wang *. (2021) RNA interference knockdown of the brassinosteroid receptor BRI1 in potato (Solanum tuberosum L.) reveals novel functions for brassinosteroid signaling in controlling tuberization. Scientia Horticultura, 290: 110516
10.Jianwei Liu#, Shufen Wang#, Hao Wang#, Bote Luo, Yiyong Cai, Xiaodong Li, Yanfeng Zhang*, Xiaofeng Wang*. (2021) Rapid generation of tomato male-sterile lines with a marker use for hybrid seed production by CRISPR/Cas9 system. Molecular Breeding, 41: 25. doi:10.1007/s11032-021-01215-2
11.Shuhua Huang#, Yuxiu Liu#, Rui Deng, Tiantian Lei, Aijuan Tian, Haihua Ren, Shufen Wang, Xiaofeng Wang*. (2021) Genome-wide identification and expression analysis of the GSK gene family in Solanum tuberosum L. under abiotic stress and phytohormone treatments and functional characterization of StSK21 involvement in salt stress. Gene, 766: 145156.
12.Shufen Wang, Tixu Hu, Aijuan Tian, Bote Luo, Chenxi Du, Siwei Zhang, Shuhua Huang, Fei Zhang，Xiaofeng Wang*. (2020) Modification of serine 1040 of SIBRI1 increases fruit yield by enhancing tolerance to heat stress in tomato. International Journal of molecular Sciences, 21(20):7681. doi:10.3390/ijms21207681.
13.Yong Yang, Jun Liu, Chuanchun Yin, Luciano de Souza Vespoli, Dongdong Ge, Yanyan Huang, Baomin Feng, Guangyuan Xu, Ana Marcia E. de A. Manh?es, Shijuan Dou, Cameron Criswell, Libo Shan, Xiaofeng Wang+, Ping He+*. (2020) RNAi-based screen reveals concerted functions of MEKK2 and CRCK3 in plant cell death regulation. Plant Physiology, 183(1):331-344. doi: 10.1104/pp.19.01555.
14.Shan Zhang, Cheng Li, Haihua Ren, Tong Zhao, Qi Li, Shufen Wang, Yanfeng Zhang, Fangming Xiao*, Xiaofeng Wang*（2020）BAK1 mediates light intensity to phosphorylate and activate catalases to regulate plant growth and development. International Journal of Molecular Sciences, 21(4):1437. doi: 10.3390/ijms21041437.
15.Shufen Wang, Jianwei Liu, Tong Zhao, Chenxi Du, Shuming Nie, Yanfeng Zhang, Siqi Lv, Shuhua Huang, Xiaofeng Wang*. (2019) Modification of threonine-1050 of SlBRI1 regulates BR signalling and increases fruit yield of tomato. BMC Plant Biology, 19(1):256. doi: 10.1186/s12870-019-1869-9.
16.Shuming Nie, Shuhua Huang, Shufen Wang, Yujiao Mao, Jianwei Liu, Ruili Ma, Xiaofeng Wang*. (2019) Enhanced brassinosteroid signaling intensity via SlBRI1 overexpression negatively regulates drought resistance in a manner opposite of that via exogenous BR application in tomato. Plant Physiology and Biochemistry, 138:36-47. doi: 10.1016/j.plaphy.2019.02.014.
17.Yanfeng Zhang, Shuhua Huang, Xuefang Wang, Jianwei Liu, Xupeng Guo, Jianxin Mu*, Jianhua Tian, Xiaofeng Wang*. (2018) Defective APETALA2 genes lead to sepal modification in Brassica crops. Frontiers in Plant Science, 9:367. doi: 10.3389/fpls.2018.00367
18.Imkampe J, Halter T, Huang S, Schulze S, Mazzotta S, Schmidt N, Manstretta R, Postel S, Wierzba M, Yang Y2, vanDongen WM, Stahl M, Zipfel C, Goshe MB, Clouse S, de Vries SC, Tax F, Wang X*, Kemmerling B* . (2017) The arabidopsis leucine-rich repeat receptor kinase BIR3 negatively regulates BAK1 receptor complex formation and stabilizes BAK1. The Plant Cell, 9: 2285-2303. doi: 10.1105/tpc.17.00376.
19.Shuhua Huang, Shuming Nie, Shufen Wang, Jianwei Liu, Yanfeng Zhang, Xiaofeng Wang*. (2017) SlBIR3 negatively regulates PAMP responses and cell death in tomato. International Journal of Molecular Sciences, 18(9):1966. doi: 10.3390/ijms18091966.
20.Shuming Nie, Shuhua Huang, Shufen Wang, Dandan Cheng, Jianwei Liu, Siqi Lv, Qi Li, Xiaofeng Wang*. (2017) Enhancing brassinosteroid signaling via overexpression of tomato (Solanum lycopersicum) SlBRI1 improves major agronomic traits. Frontiers in Plant Science, 8:1386. doi: 10.3389/fpls.2017.01386.
21.Cheng Li, Shan Zhang, Xiaofeng Wang*. (2017) Novel signaling interface constituted with membrane receptor-like kinases emerged from the study of interaction and transphosphorylation of BRI1 and BAK1. Current Topics in Medicinal Chemistry, 17(21):2393-2400. doi: 10.2174/1568026617666170414144145.
22.Qiannan Wang, Shufen Wang, Sufu Gan, Xin Wang, Jianwei Liu, Xiaofeng Wang*. (2016) Role of specific phosphorylation sites of Arabidopsis brassinosteroid-insensitive 1 receptor kinase in plant growth and development. Journal of Plant Growth Regulation, 35(3): 755-769. DOI 10.1007/s00344-016-9580-7
23.Xin Wang, Dongli Gao, Jinjing Sun, Min Liu, YaoYao Lun, Jianshu Zheng, Shenhao Wang, Qingzhi Cui, Xiaofeng Wang*, Sanwen Huang*. (2016) An exon skipping in a SEPALLATA-Like gene is associated with perturbed floral and fruits development in cucumber. Journal of Integrative Plant Biology, 58(9):766-771. doi: 10.1111/jipb.12472.
24.Shan Zhang, Cheng Li, Qi Li, Qiannan Wang, Shuhua Huang, Yanfeng Zhang and Xiaofeng Wang*. (2015) Functional divergence of BAK1 genes from Brassica rapa in regulating plant architecture. Genetics and Molecular Research, 14 (4): 14587-14596
25.Srijeet K. Mitra, Ruiqiang Chen, Murali Dhandaydham, Xiaofeng Wang, Kevin R. Blackburn, Uma Kota, Michael B. Goshe, Daniel Schwartz, Steven C. Huber, Steven D. Clouse*. (2015) An autophosphorylation site database for leucine-rich repeat receptor-like kinases in Arabidopsis thaliana. Plant Journal, 82(6):1042-60.
26.Vikramjit S. Bajwa, Xiaofeng Wang, Kevin R. Blackburn, Michael B. Goshe, Srijeet K. Mitra, Elisabeth L. Williams, Gerard J. Bishop, Sergei Krasnyanski, George Allen, Steven C. Huber, and Steven D. Clouse*. (2013) Identification and functional analysis of tomato BRI1 and BAK1 receptor kinase phosphorylation sites. Plant Physiology, 163 (1):30-42.
27.Man-Ho Oh, Xiaofeng Wang, Steven D. Clouse, and Steven C. Huber*. (2012) Deactivation of the Arabidopsis BRI1 receptor kinase by autophosphorylation within the glycine-rich loop involved in ATP binding. Proc Natl Acad Sci U S A, 109(1):327-332.
28.Man-Ho Oh, Xiaofeng Wang, Uma Kota, Michael B. Goshe, Steven D. Clouse, and Steven C. Huber*. (2009) Tyrosine phosphorylation of the BRI1 receptor kinase emerges as a component of Brassinosteroid signaling in Arabidopsis. Proc Natl Acad Sci U S A, 106 (2):658-663
29.Xiaofeng Wang, Uma Kota, Kai He, Kevin Blackburn, Jia Li, Michael B. Goshe, Steven C. Huber, Steven D. Clouse*. (2008) Sequential transphosphorylation of the BRI1/BAK1 receptor kinase complex impacts early events in Brassinosteroid signaling. Developmental Cell, 15(2):220-235
30.Xiaofeng Wang, Michael B. Goshe, Erik J. Soderblom, Brett S. Phinney, Jason A. Kuchar, Jia Li, Tadao Asami, Shigeo Yoshida, Steven C. Huber, Steven D. Clouse*. (2005) Identification and functional analysis of in vivo phosphorylation sites of the Arabidopsis BRASSINOSTEROID INSENSITIVE 1 receptor kinase. The Plant Cell, 17: 1685-1170
31.Lianhai Fu, Xiaofeng Wang, Yoram Eyal, Yimin She, Lynda J. Donald, Kenneth G. Standing, Gozal Ben-Hayyim*. (2002) A selenoprotein in the plant kingdom: mass spectrometry confirms that an opal codon (UGA) encodes selenocysteine in Chamydomonas reinhadtii glutathion peroxidase. Journal of Biological Chemistry, 277(29):25983-25991
通訊地址：陜西楊凌邰城路3號 西北農(nóng)林科技大學(xué)園藝學(xué)院
郵編：712100
電子郵箱: wangxff99@nwsuaf.edu.cn
